Human cot-1 dna®
Web在核酸复性研究中,定义了一个Cot的术语,(Co为单链DNA的起始浓度,t是以秒为单位的时间),用以表示复性速度与DNA 顺序复杂性的关系。 在探讨DNA顺序对复性速度的影响时,将温度、溶剂离子强度、核酸片段大小等其它影响因素均予以固定,以不同程度的核酸分子重缔合部分(在时间t时的复性率)取对数后对Cot作图,可以得到如图所示的曲线,用 … WebHybloc™ Competitor DNA, which is Cot-1 DNA®, is the repetitive sequence fraction of genomic DNA. Hybloc™ DNA may be effectively substituted for any other competitor DNA (i.e., Cot-1 DNA®, salmon sperm, etc.) for fluorescence in situ hybridization (FISH), Southern blots, microarray technology, or any other protocol requiring competition to ...
Human cot-1 dna®
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Web18 feb. 2024 · 人COT DNA可用于染色体原位抑制(CISS)杂交。 粘粒或YAC探针含有重复序列,其产生分布在整个染色体上的单特异性杂交信号。 为了能与染色体靶位点发生特异性杂交,将探针与过量的未标记的竞争性人COT DNA一起变性。 在微阵列分析、滤光片和荧光原位杂交实验中,人COT DNA可用于抑制与人重复序列发生非特异性杂交。 抢首赞 评 … Web人 Cot-1 DNA™ 是一种胎盘 DNA,大小主要在50至 300 bp 范围内,且富含重复 DNA 序列(如 Alu 和 Kpn 家族成员)。 每个包装的提供量足以用于 5–10 次 Southern 或500次 原位 杂交。 性能和质量检测 琼脂糖凝胶电泳验证纯度和 DNA 大小。 采用分光光度法,通过在 50 mM NaOH 中以 1:100 的比例稀释人 Cot-1 DNA™ 并使用转换因子 0.033 µg/µL/A 260 验 …
Webthermo,15279101,human cot-1 dna fluor qc 1ml at 1mg/ml fluorometry 品牌:Thermo 技术资料:0 篇 上海易汇生物科技有限公司,是一家集研发、销售为一体的高新技术生物企业,公司 Websurmised that Cot- 1 DNA is critical to ISH and can also aid in ex situ hybridization. Commercial sources of Cot-1 DNA currently exist for at least three mammalian species, i.e., hamster, human, and mouse, but are unavailable for most species, including all plants. To overcome this problem, we have developed a tech- nique that generates high ...
WebUse COT Human DNA that has been tested using DNA microarrays and comparative genome hybridization (CGH). Repetitive elements (IRS) present in a probe (e.g., … Web25 dec. 2024 · 将含双index接头封阻序列的工作液、Cot-1 DNA(Human Cot-1 DNATM-Fluorometric QC InvitrogenTM)和待杂交文库置于4℃解冻。融化后震荡混匀并离心,按照表8加入1.5mL离心管中,震荡混匀并离心。
Web公司成立至今已有近十年历史,在二代测序应用方面有着丰富的经验,拥有强有力的数据处理分析和信息挖掘技术,可提供个性化、高通量的基因组学和转录组学研究服务。
WebHuman Cot-1 DNA is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA … how to get rid of mdf sawdustWeb人 Cot-1 DNA™ 是一种胎盘 DNA,大小主要在50至 300 bp 范围内,且富含重复 DNA 序列(如 Alu 和 Kpn 家族成员)。 每个包装的提供量足以用于 5–10 次 Southern 或500次 … how to get rid of mcafee virus pop upsWebBuffer 10mM TrisHCl, 1mM EDTA, pH 7,4. # 31002 COT I Human DNA 500µg. Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing. * availibility of sample size may be limited. 209,00 € 2. 165,00 €. order / kaufen. Final price excl. shipping costs 3. how to get rid of mcafe pop upsWeb16 jan. 2024 · Cot-1,指的是Cot值为1时,经高温变性后又复性为双链的DNA。 Cot本身是描述DNA动力学特点的参数。 其公式为Cot=ct,其中c是物质的量浓度,单位是mol/L;t是时间,单位是s [2] 。 当Cot的值为1时,通过计算c和t的数值,就可以通过调整实验参数来获得双链的Cot-1 DNA了。 由于DNA复性时的热动力学特性是序列复杂度低的片段最先复 … how to get rid of mcafee popups in windows 11WebCot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing. Cot I human DNA is available with a … how to get rid of medical needlesWeb15 nov. 2000 · Hybridization was performed as previously described.34 Hybridization mixture (5 μL) that contained 3 ng/μL of the centromere 6 probe combined with 3 ng/μL of each cosmid or PAC probe, 1.5 μg human Cot-1 DNA, and 3.5 μL hybridization mix (50% formamide, 10% dextran sulphate, 50 mmol/L sodium phosphate, pH 7.0, 2 × sodium … how to get rid of mcafee webadvisorWebHuman Cot-1 DNA™-Fluorometric QC is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA. Store at -20°C. Guaranteed stable for 12 months when properly stored. … how to get rid of medical bills